Resistance of <i>Klebsiella</i> to Imipenem by Production of Carbapenemase Gene <i>bla</i><i><sub>IMP</sub></i> at Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso

Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β-lactam core antibiotics, except for monobactams. The objective this study was to identify carbapenemase gene blaIMP in genus Klebsiella at Charles De Gaulle Pediatric University Hospital (CHUP-CDG) Ouagadougou, Burkina Faso. Methods: involved 17 strains responsible human infection and isolated from various biological samples during period 2009 2013. were tested antimicrobial susceptibility cefotaxime, ceftazidime imipenem using Mueller-Hinton agar diffusion method. resistance genes detected by conventional PCR specific primers molecular biology laboratory CERBA/LABIOGENE, Results: antibiotic test showed high isolates cephalosporins. A cefotaxime-resistance rate (82.35%) ceftazidime-resistance (88.23%) found among against 11.76% imipenem. Analysis products gel electrophoresis revealed 4 (23.53%) with blaIMP-type gene. Conclusion: is a well-known bacterium clinical practice. present demonstrated cephalosporin-resistant CHUP-CDG. More effective monitoring treatment solutions needed prevent spread these resistant strains.